Figure 7
From: Inhibition of human lung cancer cell proliferation and survival by wine
Effect of white wine on Akt in A549 NSCLC cells. Total cell lysates were prepared from A549 cells that were serum-deprived for 24 h then treated with or without 2% or 5% white wine (Riesling) for 24 h. Cell lysates (25 μg) were resolved by SDS-PAGE and immunoblotted with anti-phospho or anti-total- Akt antibody. Representative immunoblots of 2 independent experiments are shown. Immunoblots were scanned to quantitate the density of the bands and the results in the bar graphs are mean ± SE. All values are arbitrary densitometry units expressed relative to untreated control.