Figure 4

Expression levels of IFNgR1 and IFNgR2 subunits of the IFNg receptor complex do not increase in four different tumor cell lines after PMA treatment. Cells were incubated in culture medium contaning 10³ ng/ml PMA and 172 mM ethanol for 48 hr. Live (propidium iodide negative) cells were used to measure cell surface expression of IFNg receptors. Fixed and permeabilized cells were used to measure total expression of IFNg receptors (both cytoplasmic and cell surface). Mean values of two independent experiments are plotted.