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Fig. 4 | Cancer Cell International

Fig. 4

From: Long non-coding RNA CCL14-AS suppresses invasiveness and lymph node metastasis of colorectal cancer cells by regulating MEP1A

Fig. 4

CCL14-AS interacts with MEP1A mRNA and reduces its stability. A FISH assay was conducted to determine the subcellular location of CCL14-AS in SW620 cells. DAPI‑stained nuclei are blue. Scale bar: 20 μm. B–D RNA fraction assay and online software LncLocator confirmed the subcellular location of CCL14-AS. B Western blot analysis detection of the efficiency of nuclear and cytosolic fractionation. C Nuclear fractionation analyses and qRT-PCR analyses of CCL14-AS expression in the nucleus and cytoplasm. D LncLocator predicts the localization of CCL14-AS in cell. E Gene ontology (GO) analyses of differentially expressed genes from RNA-seq assay of stable CRC cells expressing vector or CCL14-AS. F–G Heatmap (F) and volcanic map (G) showed significant differentially expressed genes after RNA-seq screening. H qRT-PCR confirmed that after overexpressing of CCL14-AS, the mRNA levels of MEP1A decreased, while knockdown of CCL4-AS increased the MEP1A expression. I–J Western blot analysis I and immunofluorescence assay (J) both confirmed that overexpressing CCL14-AS reduced MEP1A protein level. Scale bar: 25 μm. K MS2-RIP experiments detect the direct binding of CCL14-AS to MEP1A mRNA in CRC cells. L RNA-stability analysis showed that CCL14-AS promote the degradation of MEP1A mRNA. ** p < 0.01, *** p < 0.001

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Cancer Cell International

ISSN: 1475-2867

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