Fig. 1

Hypoxia enhances erastin-induced ferroptosis in nasopharyngeal carcinoma cells. (A) HNE1 and 6-10B cells were treated with 0, 2.5, 5, 10, 20 µM erastin for 24 h under hypoxia or normal control culture environment, cell viability was assessed by CCK-8 assay. (B-G) HNE1 and 6-10B cells were treated with 10 µM erastin under hypoxia or normoxia 24 h. (B) Cell floating and death were evaluated via observation using a microscope. (C) ROS concentration was assessed by flow cytometer presented by MEAN FITC. (D) Intracellular lipid ROS was measured by using an imaging probe that becomes green fluorescent upon activation by lipid ROS. (E) MDA concentration was assessed by MDA assay. (F) GSH concentration was assessed by GSH assay. (G) Fe concentration was assessed by Fe assay. The data are presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001