Fig. 5

Hypoxia-induced BAP1 decreased SLC7A11 expression by stabilizing H2A. (A) BAP1 knockdown 6-10B cells were subjected to hypoxia or normal stimulation. BAP1 and H2A expression was detected by representative immunoblotting analysis. (B) 6-10B cells (shBAP1 or shNC) were subjected to MG132 or solvent 3 h before harvest, BAP1 and H2A expression was detected by representative immunoblotting analysis. (C) 6-10B cells were subjected to hypoxia 24 h. And BAP1 antibody or normal rabbit IgG antibody were subjected to co-IP, then the indicated antibodies were used for immunoblotting analysis. (D) 6-10B cells transfected with BAP1 knockdown lentiviruses or empty virus were subjected to hypoxia 24 h and treated with MG132 3 h before harvest. And H2A antibody or normal rabbit IgG antibody were subjected to co-IP, then the UB antibody was used for immunoblotting analysis. (E) 6-10B cells transfected with BAP1 knockdown lentiviruses or empty virus were subjected to hypoxia or normal stimulation. SLC7A11 transcription level was detected by qRT-PCR. The data are presented as mean ± SD. *P < 0.05; **P < 0.01