Fig. 4

Effect of B[a]P and/or EtOH exposure on EMT makers in cervical cancer cells. (A) Effect of B[a]P and/or EtOH exposure on expression on EMT markers in cervical cancer cells as determined by qRT-PCR. Briefly, cervical cancer cells were treated with B[a]P and/or EtOH for 48 h. RNA was isolated and cDNA was prepared and subjected to qRT-PCR for EMT markers analysis. Bar graphs represent relative mRNA fold expression of E-cadherin, N-cadherin, Snail, Zeb1, vimentin, MMP-2 and MMP-9 in CaSki and SiHa (mean ± SEM). (B) B[a]P and/or EtOH exposure in CaSki and SiHa cells modulates the expression of EMT markers on protein level as determined by Western blot analysis. Asterisk (*) denotes the significant value p < 0.05. Each experiment has been repeated three times