Fig. 6

B[a]P and/or EtOH exposure regulates inflammation mediators in cervical cancer cells. (A) Effect of B[a]P and/or EtOH exposure on mRNA expression of inflammation modulators COX-2, TNF-α, VEGF and IL-6 in CaSki and SiHa cells as determined by qRT-qPCR analysis. The cells were exposed with B[a]P and/or EtOH for 48 h. (B, D) Effect of B[a]P and/or EtOH and NF-κB inhibitor on IL-6 and, COX-2 expression. The cervical cancer cells were pretreated with 25 µM NF-κB inhibitor for 24 h followed by 48 h exposure of B[a]P and/or EtOH. Expression of IL-6 and COX-2 mRNA expression was measured by qRT-PCR. (C) B[a]P and/or EtOH exposure increased the expression of oncogenic IL-6, which was decreased effectively by Cur/PLGA-Cur in cervical cancer cells CaSki and SiHa. IL-6 expression was analyzed using a cytokine assay kit. Asterisk (*) denotes the significant value p < 0.05. Each experiment has been repeated three times