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Fig. 2 | Cancer Cell International

Fig. 2

From: Adipose stem cell exosomes, stimulated by pro-inflammatory factors, enhance immune evasion in triple-negative breast cancer by modulating the HDAC6/STAT3/PD-L1 pathway through the transporter UCHL1

Fig. 2

Extraction and identification of exosomes derived from ADSCs. A Representative images of Oil-red O-stained ADSCs. Scale bar = 25 μm. B Expression levels of PPAR-γ, HSL, LPL, ADIPOQ, and FABP4 mRNAs in the ADSCs on Day 0 and Day 7. C Representative TEM images of the exosomes. Scale bar = 100 nm. D Size of the exosomes by NTA. E Immunoblot showing levels of CD9, CD63 and CD81 proteins in the ADSCs and exosomes. F Representative images showing the distribution of exosomes (PKH26) in the TNBC cells. Scale bar = 25 μm. G UCHL1 protein expression in the ADSCs, ADSCs-con and ADSCs-si-UCHL1. H UCHL1 protein expression in the Exos, Exos-con and Exos-si-UCHL1. I The transfection efficiency of GFP-oe-NC and GFP-oe-UCHL1 in ADSCs. J UCHL1 protein expression in Exos-GFP-oe-NC and Exos-GFP-oe-UCHL1 transfection. *P < 0.05 vs. Day 0. #P < 0.05 vs. ADSCs-con

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Cancer Cell International

ISSN: 1475-2867

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