Fig. 3

ADSCs-Exos^{IFN−γ+TNF−α} promoted immune escape of TNBC cells by transporting UCHL1. ADSCs were treated with 10 ng/mL IFN-γ and TNF-α, and transfected with si-NC or si-UCHL1. The exosomes were extracted from the supernatant. TNBC cells were treated with 10 μg/mL of the ADSCs-CM and different exosomes for 24 h, and then co-cultured with THP-1 cells for 48 h or with T cells for 16 h. A UCHL1 protein levels in TNBC cells in the indicated groups. B Viability of TNBC cells in the indicated groups. C Representative images showing Ki-67 expression in TNBC cells in the indicated groups. Scale bar = 100/25 μm. D Representative images showing migration of TNBC cells in the indicated groups. Scale bar = 100 μm. ^*P < 0.05 vs. MDA-MB-231 or MDA-MB-468. ^#P < 0.05 vs. Exos + MDA-MB-231 or Exos + MDA-MB-468. ^&P < 0.05 vs. Exos^{IFN−γ+TNF−α+con} + MDA-MB-231 or Exos^{IFN−γ+TNF−α+con} + MDA-MB-468. E The proportion of CD68⁺CD206⁺ THP-1 cells in the indicated groups. F Arg-1, CD206, TNF-α, and iNOS expression in THP-1 cells. ^*P < 0.05 vs. THP-1. ^#P < 0.05 vs. MDA-MB-231 + THP-1 or MDA-MB-468 + THP-1. ^&P < 0.05 vs. Exos + THP-1. ^@P < 0.05 vs. Exos^{IFN−γ+TNF−α+con} + THP-1. G The proportion of CD3⁺CD8⁺ T cells in the indicated groups. H Perforin and GZMB levels in T cells. ^*P < 0.05 vs. T cells. ^#P < 0.05 vs. MDA-MB-231 + T cells or MDA-MB-468 + T cells. ^&P < 0.05 vs. Exos + T cells. ^@P < 0.05 vs. Exos^{IFN−γ+TNF−α+con} + T cells