Fig. 5

shCTCF-2 exhibited the best efficiency in lowering CTCF expression. qRT-PCR was used to detect the CTCF expression after knocking down CTCF in A HEC-1-A and B Ishikawa cell line. WB assay was used to detect the CTCF expression after knocking down CTCF in C HEC-1-A and D Ishikawa cell line. n = 3 biologically independentsamples. ^** p < 0.01, ^***p < 0.001