Fig. 6

Effect of CTCF gene knockdown on EC cells. Knocking down CTCF inhibited A invasion and B metastasis ability of HEC-1-A and Ishikawa cell lines. C Knocking down CTCF promoted HEC-1-A and Ishikawa cell apoptosis. D Knocking down CTCF promoted HEC-1-A percentage of NK cell. Each experiment was repeated three times. E Knocking down CTCF significantly inhibited tumor growth (n = 5, One mouse out of six was not included in the final count because it did not meet the preset experimental criteria and died.). ^*p < 0.05, ^**p < 0.01, ^***p < 0.001