Fig. 10

Comparative analysis of gene expression levels in tumor tissues of control, AMD3100, and A1-treated mice. The chart illustrates the relative expression levels of (A) CXCR4, (B) FGF, (C) VEGF, (D) IL-10, and (E) TGF-β genes within tumor tissues derived from three distinct groups: the control group (untreated), the AMD3100-treated group, and the A1-treated group of mice. Gene expression levels were quantified using qRT-PCR, and all experiments were performed in triplicate. The data is presented as Mean ± SEM, reflecting the variability among biological replicates. Statistical significance between the groups is denoted by *P < 0.05 and **P < 0.01, underscoring the differential expression of genes induced by AMD3100 and A1 treatments compared to the control group. These findings elucidate the modulatory effects of the treatments on critical genes implicated in tumor progression, angiogenesis, and immune regulation. Error bars reflect the standard error of the mean, thereby highlighting the consistency of the experimental outcomes