Fig. 9

Flow cytometry analysis of Treg distribution in the TME and spleen of treated mice. The diagrams illustrate the percentage of T regulatory cells (Tregs) in the (A) tumor microenvironment (TME) and (B) spleen of mice administered with the pharmacological agents A1 and AMD3100, as compared to untreated control groups. Flow cytometry was utilized to evaluate Treg populations, employing specific gating strategies to identify CD4⁺CD25⁺FoxP3⁺ cells. The experiments were triplicate, with data presented as Mean ± Standard Deviation (SD). Statistical significance is denoted by **P < 0.01 and ***P < 0.001, highlighting the variations in Treg distribution between treatment and control groups. These findings demonstrate the influence of A1 and AMD3100 on the modulation of the immune landscape within the TME and systemic lymphoid organs. Error bars represent the standard deviation among replicate experiments, ensuring consistency and reproducibility of the data