Fig. 4

Validation of proteins and assessment of the collective diagnostic efficacy of five biomarkers at the tumor tissue level (A) Western blot and average data (B) showing TIMP1, FN1, MMP11, GPNMB, and ECM1 protein levels in tumor tissues from WDLPS and DDLPS patients. n = 6 for each group. (C) HE staining, picrosirius red, and Masson staining showing tumor morphology and collagen deposition in DDLPS and WDLPS tissues. Immunohistochemical staining of TIMP1, FN1, MMP11, GPNMB, and ECM1 in WDLPS and DDLPS tissues. Scale bar = 100 μm; n = 6 for each group. (D) Statistical analysis of the five genes transcriptomic changes in WDLPS and DDLPS; n = 19 for the WDLPS group and n = 29 for the DDLPS group; log₂FC > 0 indicates that gene expression increased, and log₂FC < 0 indicates that gene expression decreased. (E-J) ROC curve of TIMP1, FN1, MMP11, GPNMB, ECM1, and the combination of five markers for WDLPS and DDLPS tumor tissues