Fig. 4

miR-615-3p directly targets SESN2 in OS cells. A. Protein levels of total and phosphorylated mTOR in different groups (anti-NC and anti-miR-615-3p). B. Venn diagram showing the predicted mTORC1-related target genes of miR-615-3p obtained from StarBase databases. C and D. The mRNA expression patterns of the predicted target genes in different groups (anti-NC and anti-miR-615-3p). E. The wild-type and the mutated sequences of the SESN2 mRNA 3’-UTR (mutation site: red). F and G. The luciferase activity of the OS cells (143B and MNNG-HOS) in luciferase reporter plasmid containing wild-type SESN2 3’-UTR (SESN2-WT) and mutant SESN2 3’-UTR (SESN2-MUT) co-transfected with miR-615b-3p inhibitors or negative control was assessed. H and I. RIP assays using antibodies against AGO2 or IgG were performed in cellular lysates from 143B and MNNG-HOS cells. qRT-PCR showed the relative enrichment of SESN2 in different groups (anti-NC and anti-miR-615-3p). Results are displayed as mean ± SD, * indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001