Fig. 4

Small molecule-mediated inhibition of CK2 affects the expression of PPARγ. (A) RT-qPCR analysis of SCD-1 gene expression in ccRCC cells treated with vehicle (DMSO, Control) or 10 µM CX-4945 for 24 h. TATA box binding protein (TBP)-coding gene was employed as reference gene. Values on the Y-axis are expressed as the ratio between the level of the gene of interest and the reference gene. Experiments were performed three times in triplicates. Average values are shown +/- STDEV. (B) RT-qPCR analysis of SCD-1 gene expression in ccRCC cells heterotransplanted into nude mice treated with vehicle or Silmitasertib sodium salt (n = 4 per group). (C) Analysis of the mRNA levels of the indicated transcription factors expressed in cells treated as indicated in (A). (D) Whole cell lysates from cells treated as in (A) were analysed by Western blot probing the membrane with anti-PPARγ antibody. β-actin detection was used as loading control