Fig. 5
From: Organoids as a model system for researching human neuroendocrine tumor of the breast
Single-cell RNA-seq analysis of the NETB patient-derived organoids. (A) The t-SNE plot showing 17 clusters. The bar chart showing the proportion of each cluster. Each cluster was shown in a different color. (B) The t-SNE plot showing 3 cell types. The bar chart showing the proportion of each type. Each type was shown in a different color. (C) The heatmap separately displayed the expression proportions and relative expression levels of marker genes in three clusters. (D) The images of the ESR1, ESR2, PGR and ERBB2 expression. (E) The pseudotime trajectory analysis generated a single-branch trajectory that depicted the tumor’s development. (F) Different cell types in the trajectory were marked with different colors. (G) Heatmap showing all cells along the pseudo-time, which was clustered into three profiles. Color key differentially coding from blue to red indicated the relative expression levels from low to high. (H) The GO enrichment dot plot showing enriched pathways on the vertical axis and the ratio of differentially expressed genes annotated to GO terms to the total number of differentially expressed genes on the horizontal axis. The size of the dots represents the number of genes, and the color gradient from red to blue indicates the level of enrichment significance, with red representing higher significance and blue lower significance. (I) The KEGG enrichment dot plot displayed enriched pathways on the vertical axis and the ratio of differentially expressed genes annotated to KEGG pathway IDs to the total number of differentially expressed genes on the horizontal axis. The size of the dots indicates the number of genes, while the color gradient from red to blue represents the enrichment significance, with red indicating higher significance and blue indicating lower significance. (J) The network structure diagram corresponding to the protein-protein interaction analysis