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Fig. 8 | Cancer Cell International

Fig. 8

From: Predicting hepatocellular carcinoma outcomes and immune therapy response with ATP-dependent chromatin remodeling-related genes, highlighting MORF4L1 as a promising target

Fig. 8

MORF4L1 activates the Hedgehog pathway to promote stemness in HCC. A Volcano plot depicting differential gene expression (DEGs) between groups with high and low MORF4L1 expression in TCGA-LIHC. B and C Pathways identified from GSEA analysis of DEGs. D Western blot analysis for critical Hedgehog pathway proteins in HCC cells with MORF4L1 knockdown or overexpression. E Representative images of spheroids by indicated cells. Scale bar: 50 μm. F RT-PCR analysis for relative mRNA expression levels of CSC markers in indicated cells. G Limiting dilution assay reveals sphere formation frequency in indicated cells. H and I Assessments of plate colony formation and transwell migration/invasion assays in indicated cells. J Growth curves of subcutaneous xenograft tumors in nude mice injected with indicated HCC cells. Data are presented as means ± SD. (*p < 0.05; **p < 0.01; *p < 0.001)

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Cancer Cell International

ISSN: 1475-2867

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