Fig. 5
HnRNPC enhances XB130 mRNA stability and translation efficiency by binding to the 3’UTR of XB130 mRNA. A: mRNA decay assays analyzed the stability of XB130 mRNA in control and hnRNPC-silenced cells. B-C: Dual-luciferase reporter recombinant vectors containing the 3’UTR segment 113–230 (B) or 503–660 (C) of XB130 mRNA were transfected into control and hnRNPC-silenced cells, followed by mRNA decay assays to assess the stability of the reporter gene hRluc mRNA. D: RIP assays were performed to examine interactions between eIF4E protein and XB130 and Hsp90 mRNA in control and hnRNPC-silenced cells. IgG-bound RNA was used as a negative control. E: Co-IP experiments were conducted to investigate interactions of hnRNPC with eIF4E and hnRNPR in hnRNPC-overexpressing cells, under RNA-present and RNA-absent conditions. IgG-bound protein was used as a negative control. t1/2: half‑lives of mRNA; ActD: actinomycin D. * p < 0.05, ** p < 0.01, *** p < 0.001