Fig. 7
Silencing XRN1 or DIS3L2 reverses the effects of hnRNPC silencing on XB130 mRNA stability. A-B: NC siRNA, XRN1 siRNA (A), or DIS3L2 siRNA (B) were transfected into control and hnRNPC-silenced cells. mRNA decay assays analyzed the stability of XB130 mRNA in these groups. C-D: Dual-luciferase reporter recombinant vectors containing the 3’UTR segment 113–230 of XB130 mRNA were co-transfected with NC siRNA, XRN1 siRNA (C), or DIS3L2 siRNA (D) into control and hnRNPC-silenced cells, followed by mRNA decay assays to assess the stability of the reporter gene hRluc mRNA in cells. E-F: Dual-luciferase reporter recombinant vectors containing the 3’UTR segment 503–660 of XB130 mRNA were co-transfected with NC siRNA, XRN1 siRNA (E), or DIS3L2 siRNA (F) into control and hnRNPC-silenced cells. mRNA decay assays were conducted to evaluate the stability of the reporter gene hRluc mRNA in cells. * means significantly different from the control group (NC siRNA + NC shRNA); # means significantly different from the hnRNPC-silenced group (NC siRNA + hnRNPC shRNA-2). t1/2: half‑lives of mRNA; ActD: actinomycin D. * p < 0.05, ** p < 0.01, *** p < 0.001, #p < 0.05, ##p < 0.01, ###p < 0.001