Heterogeneous nuclear ribonucleoprotein C promotes non-small cell lung cancer progression by enhancing XB130 mRNA stability and translation

Table 1 Comparison of luciferase activity to mRNA content in NSCLC cells

Group	Luciferase level NCI-H292 /PC-9	mRNA level NCI-H292 /PC-9	Ratio¹ NCI-H292 /PC-9
NC shRNA + psiCHECK (113–230)	1.000 ± 0.105 / 1.000 ± 0.052	1.003 ± 0.093 / 1.000 ± 0.062	0.993 ± 0.105 / 0.997 ± 0.055
hnRNPC shRNA-1 + psiCHECK (113–230)	0.184 ± 0.004* / 0.497 ± 0.058*	0.433 ± 0.025* / 0.670 ± 0.017*	0.430 ± 0.010*** / 0.743 ± 0.081*
hnRNPC shRNA-2 + psiCHECK (113–230)	0.247 ± 0.007* / 0.507 ± 0.085*	0.717 ± 0.047 / 0.737 ± 0.074	0.343 ± 0.012* / 0.687 ± 0.119
NC shRNA + psiCHECK (503–660)	1.000 ± 0.004 / 0.997 ± 0.045	1.000 ± 0.040 / 1.000 ± 0.066	0.997 ± 0.006 / 0.997 ± 0.045
hnRNPC shRNA-1 + psiCHECK (503–660)	0.653 ± 0.003* / 0.610 ± 0.095*	0.840 ± 0.046 / 0.810 ± 0.020	0.777 ± 0.006*** / 0.753 ± 0.118*
hnRNPC shRNA-2 + psiCHECK (503–660)	0.603 ± 0.002* / 0.573 ± 0.047*	0.730 ± 0.010* / 0.707 ± 0.021*	0.827 ± 0.006*** / 0.813 ± 0.064*

¹ A ratio of 1.0 denotes a direct and proportional relationship between luciferase activity and the corresponding mRNA levels. Ratios above 1 indicate that the silencing of hnRNPC enhances the translation of luciferase mRNA, while ratios below 1 signify that silencing hnRNPC suppresses the translation of luciferase mRNA. The greater the ratio deviates from 1, the more pronounced the enhancement or suppression effect. * p < 0.05, * p < 0.01, *** p < 0.001

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