Fig. 3
From: Senescence-related circRNA circHIF-1α is associated with pancreatic cancer progression
CircHIF-1α delays senescence and promotes the proliferation and mobility of PC cells in vitro. (A) Effectiveness of lentivirus transfection of sh-circHIF-1α verified by RT-qPCR. (B) SA-β-Gal staining assay was performed to determine the senescent PC cells with circHIF-1α knockdown. (C) Immunofluorescence experiment verified the effect of circHIF-1α on the expression of senescence-related biomarker including laminb1 and γ-H2AX. (D) Western blotting was performed to examine the expression of senescence-related protein levels, including RB, laminb1, γ-H2AX and P21. (E) The CCK-8 assay was used to determine the proliferation of PC cells with circHIF-1α loss. (F) Colony formation assay was used to detect the number of colonies in the circHIF-1α-knockdown, and negative control groups. (G) EDU assay was used to determine the EDU positivity of PC cells with circHIF-1α- knockdown. (H-I) Wound healing and transwell assays were used to determine the migration and invasion of PC cells with circHIF-1α- knockdown. (J) Western blotting analysis of EMT markers, including N- cadherin, E-cadherin and vimentin expression, in each group of PC cells. *p < 0.05; **p < 0.01