Fig. 7
From: Senescence-related circRNA circHIF-1α is associated with pancreatic cancer progression
CircHIF-1α delayed senescence and promoted the proliferation and motility of PC cells in a miR-375- and HUR-dependent manner. The rescue effects of miR-375 mimic and HUR knockdown on circHIF-1α- overexpressing lentivirus. (A) Total cells and β-Gal staining positive cells were counted, and the percentage of β-Gal staining positive cells was calculated in each group of PC cells. (B) Immunofluorescence experiments verified the expression of senescence-related biomarkers in each group, including laminb1 and γ-H2AX. (C) The expression of RB, laminb1, γ-H2AX and P21 was examined using western blotting in each group. (D) The CCK-8 assay was used to determine the rate of PC cell proliferation in each group. (E) Colony formation assay was used to detect colony formation in each group. (F) EDU assay was performed to detect the rate of EDU positivity in each group. (G-H) Wound healing and transwell assays were used to determine the rates of migration and invasion in each group. (I) The expression of EMT markers was examined using western blotting in each group. *p < 0.05; **p < 0.01