Fig. 2

Neural gene expression differs in DAOY cells grown as adherent monolayers and in cells cultured as spheres. Quantitative RT-PCR analysis of mRNA levels of PROM1, SOX2, MAP2, DLX2, TUBB3 and CSPG4 genes in adherent DAOY cells and DAOY spheres two and three days after seeding. GAPDH was used as a reference gene. Each column represents the mean of five independent experiments with error bars indicating standard deviation. The level of statistical significance: * p < 0.05, ** p < 0.01, *** p < 0.001