Table 5 The impact of the frameshift mutation c. 145* insertion A in the NRAS gene on the protein structure was determined using mutation taster
S.No | Start (aa) | End (aa) | Protein features | Details | Impact of fameshift mutation on NRAS protein |
|---|---|---|---|---|---|
1 | 49 | 57 | STRAND | Lost | |
2 | 57 | 61 | NP_BIND | GTP | Lost |
3 | 62 | 64 | HELIX | Lost | |
4 | 66 | 74 | HELIX | Lost | |
5 | 76 | 83 | STRAND | Lost | |
6 | 87 | 104 | HELIX | Lost | |
7 | 89 | 89 | MOD_RES | Phosphoserine; by STK19 | Lost |
8 | 89 | 89 | MUTAGEN | S-> A: Abolished phosphorylation by STK19 | Lost |
9 | 111 | 116 | STRAND | Lost | |
10 | 116 | 119 | NP_BIND | GTP | Lost |
11 | 127 | 137 | HELIX | Lost | |
12 | 141 | 143 | STRAND | Lost | |
13 | 146 | 148 | TURN | Lost | |
14 | 152 | 166 | HELIX | Lost | |
15 | 164 | 164 | MUTAGEN | R-> A: Loss of GTP-binding activity | Lost |
16 | 166 | 185 | REGION | Hypervariable region | Lost |
17 | 170 | 170 | CROSSLNK | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | Lost |
18 | 181 | 181 | MUTAGEN | C-> S: Loss of plasma membrane localization | Lost |
19 | 181 | 181 | LIPID | S-palmitoyl cysteine | Lost |
20 | 186 | 186 | LIPID | S-farnesyl cysteine | Lost |
21 | 187 | 189 | PROPEP | Removed in mature form /id="PRO_0000043007” | Lost |