Fig. 6
Immunohistochemical evaluation of the expression of hERG1, hERG1/β1, nNaV1.5 and NHE1 in human primary BCa. IHC experiments and assessment of score were performed as reported in the Materials and Methods section. Representative sections of healthy breast for each marker (A). Original magnification ×200. Scale bar: 100 μm. Representative examples of Score 1, Score 2 and Score 3 are reported for hERG1 (B), nNav1.5 (C), hERG1/β1 integrin complex (D) and NHE1 (E). Original magnification ×200. Scale bar: 100 μm. (F) Concomitant expression of hERG1, hERG1/β1 integrin complex, nNav1.5 and NHE1 in the same cells of a representative sample. Original magnification ×400. Scale bar: 50 μm. (G) Heatmap showing the Spearman Correlation Coefficient for hERG1/β1 integrin complex, nNav1.5 and NHE1. All the P values are statistically significant (P < 0.0001)