Fig. 4

NQO1 Promotes anoikis resistance of CRC cells. (A-B) GO pathway analysis and GeneMANIA found that NQO1 may participate in biological processes and the correlation between NQO1 and apoptosis-related protein. (C) The effect of differential expression of NQO1 on apoptosis of CRC cells was detected by Hoechst 33,342 staining. (D) The effect of differential expression of NQO1 on apoptosis of CRC cells was detected by Annexin V-PE/7-AAD assay using flow cytometry. (E) Differential expression of NQO1 on HCT-116 and DLD-1 cells were cultured successfully and photographed under microscope. (F) Western blot determination of apoptosis markers protein expression in CRC cells after differential expression of NQO1