Fig. 6

miR-485-5p directly targeted NQO1. (A) Venn diagrams showed the overlap from four independent databases and found possible candidate upstream regulators of NQO1. (B) qRT-PCR showed the miR-485-5p mRNA expression transfection of miR-485-5p mimic and miR-485-5p inhibitor. (C) qRT-PCR and Western blot showed the expression levels of NQO1 mRNA and protein after transfection of miR-485-5p mimic and miR-485-5p inhibitor. (D) The binding sites of 3’-UTR and miR-485-5p. Verification of the effect of miR-485-5p targeting NQO1 by Luciferase reporter assay. *P < 0.05, * * P < 0.01