Fig. 7

The miR-485-5p/NQO1 regulated aerobic glycolysis in CRC. (A) Effects of miR-485-5p/NQO1 axis on glucose uptake, lactate and ATP production in miR-485-5p inhibitor or miR-485-5p mimic and miR-485-5p inhibitor + sh-NQO1 or miR-485-5p + NQO1 cells. (B) Effects of miR-485-5p/NQO1 axis on the proliferation of miR-485-5p inhibitor or miR-485-5p mimic and miR-485-5p inhibitor + sh-NQO1 or miR-485-5p + NQO1 cells was detected by Colony formation assay. (C) Effect of miR-485-5p/NQO1 axis on the migration ability of miR-485-5p inhibitor or miR-485-5p mimic and miR-485-5p inhibitor + sh-NQO1 or miR-485-5p + NQO1 cells was detected by wound healing assay. (D) Effect of miR-485-5p/NQO1 axis on the migration ability of miR-485-5p inhibitor or miR-485-5p mimic and miR-485-5p inhibitor + sh-NQO1 or miR-485-5p + NQO1 cells was detected by transwell assays. (E) Effect of miR-485-5p/NQO1 axis on EMT, apoptosis and glycolysis related marker proteins in miR-485-5p inhibitor or miR-485-5p mimic and miR-485-5p inhibitor + sh-NQO1 or miR-485-5p + NQO1 cells was detected by Western blot. *P < 0.05, * * P < 0.01