Fig. 3
From: Rack1 promotes breast cancer stemness properties and tumorigenesis through the E2F1-SOX2 axis
Rack1 knockdown inhibits SOX2 expression in breast cancer cells. (A-B) qRT-PCR and Western blot were performed to determine the mRNA (A) and protein (B) expression levels of Rack1 and stemness-related genes in control or Rack1 knockdown MCF7 and MDA-MB-231 cells. (C) The dual-luciferase reporter assay was used to determine the transcriptional activity of SOX2 in control and Rack1 knockdown breast cancer cells. (D) Correlation analysis of Rack1 and SOX2 expression in 31 cases of breast cancer tissue. (E) Western blotting and qRT-PCR analysis of the expression levels of Rack1 and SOX2 in control, Rack1-silenced breast cancer cells and after exogenous overexpression of SOX2 in Rack1-silenced cells. (F) Effect of SOX2 overexpression on the sphere-forming ability in Rack1-silenced breast cancer cells. All data are shown as the number of mammospheres per 1000 cells. The data presented are expressed as mean ± SD, with statistical significance indicated as *P < 0.05, **P < 0.01, and ***P < 0.001