Fig. 5
From: Rack1 promotes breast cancer stemness properties and tumorigenesis through the E2F1-SOX2 axis
Rack1 controls breast cancer stemness through the E2F1-SOX2 Axle in BCCs. (A) Expression levels of SOX2 and E2F1 proteins in control and Rack1 knockdown MCF7 and MDA-MB-231 cells. (B) The effect of E2F1 overexpression on SOX2 protein levels in Rack1-silenced breast cancer cells was analyzed by Western blotting. (C) The effect of E2F1 overexpression on the transcriptional activity SOX2 in Rack1-silenced breast cancer cells was analyzed by dual-luciferase assay. (D-E) The effect of E2F1 overexpression on the sphere-forming ability of Rack1-silenced breast cancer cells