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Fig. 1 | Cancer Cell International

Fig. 1

From: CircRNA hsa_circ_0004781 promoted cell proliferation by acting as a sponge for miR-9-5p and miR-338-3p and upregulating KLF5 and ADAM17 expression in pancreatic ductal adenocarcinoma

Fig. 1

Identification of hsa_circ_0004781 in PDAC cells. (A) Schematic illustrating the canonical and back-splicing of PCNX1 pre-mRNA, presenting three paired primer annealing sites for PCNX1 mRNA and hsa_circ_0004781. (B) Total RNA from ASPC-1 and BXPC-3 cells was extracted, and hsa_circ_0004781 (c4781) was amplified through RT-PCR by using divergent primers, followed by agarose gel electrophoresis. (C) The hsa_circ_0004781 band was extracted from the gel, and the sequence of the BSJ region was determined through Sanger sequencing. F, forward strand; R, reverse strand. (D) cDNA and gDNA prepared from AsPC-1 and BxPC-3 cells were used to amplify the BSJ region of hsa_circ_0004781 and exon 2 of the PCNX1 gene by using divergent primers and convergent primer 1, respectively. (E) Total RNA extracted from AsPC-1 cells treated with or without RNase R was used to amplify the BSJ region of hsa_circ_0004781 and exon 2 of PCNX1 mRNA through RT-PCR with divergent primers and convergent primer 2, respectively

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Cancer Cell International

ISSN: 1475-2867

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