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Fig. 3 | Cancer Cell International

Fig. 3

From: CircRNA hsa_circ_0004781 promoted cell proliferation by acting as a sponge for miR-9-5p and miR-338-3p and upregulating KLF5 and ADAM17 expression in pancreatic ductal adenocarcinoma

Fig. 3

Effects of the knockdown and overexpression of hsa_circ_0004781 on the cell migration and expression of EMT-related marker genes in PDAC cells. (A) AsPC-1 cells were transfected with control siRNA (si-Ctr) or hsa_circ_0004781 siRNA (si-c4781) for 24 h, and the number of migrating cells was determined by performing a wound healing assay. A representative photo and quantified data are presented. Each value represents the mean ± SE of three independent experiments. **p < 0.01 vs. si-Ctr. (B-E)AsPC-1 and BxPC-3 cells were transfected with (B, D) control siRNA (si-Ctr) or si-c4781 and (C, E) the control vector (OE-Ctr) or the pcDNA-ZKSCAN-c4781 plasmid (OE-c4781) for 24 h. (B, C) Total proteins were extracted, and protein expression was determined through a Western blot assay. (D, E) Total RNA was extracted, and mRNA expression was determined through RT-qPCR. Values are presented as the mean ± SE from three independent experiments. *p < 0.05, #p < 0.001, ##p < 0.0001 vs. si-Ctr or OE-Ctr. E-cad, E-cadherin; Vim, vimentin

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Cancer Cell International

ISSN: 1475-2867

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