Fig. 5

Effects of miR-9-5p and miR-338-3p mimics on luciferase mRNA degradation and PDAC cell proliferation. (A) The 3′ UTR of luciferase mRNA contained predicted miR-9-5p and miR-338-3p target sequences from hsa_circ_0004781 (c4781). (B) AsPC-1 and BxPC-3 cells were cotransfected with the pmirGLO-c4781 reporter plasmid and either the control miRNA mimic (Ctr mimic), miR-9-5p mimic (miR9 mimic), or miR-338-3p mimic (miR338 mimic) for 24 h. Total cell lysates were collected. Luciferase activity was measured using a dual luciferase assay kit, and values were normalized to Renilla luciferase activity. Each value represents the mean ± SE of three independent experiments. ^##p < 0.0001 vs. the Ctr mimic. (C) AsPC-1 and BxPC-3 cells were transfected with the control miRNA mimic (Ctr mimic), miR-9-5p mimic (miR9 mimic), or miR-338-3p mimic (miR338 mimic) for 1–3 days. Cell proliferation was determined by performing an MTT assay. Values are presented as mean ± SE. ^##p < 0.0001, compared with the Ctr mimic