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Fig. 6 | Cancer Cell International

Fig. 6

From: CircRNA hsa_circ_0004781 promoted cell proliferation by acting as a sponge for miR-9-5p and miR-338-3p and upregulating KLF5 and ADAM17 expression in pancreatic ductal adenocarcinoma

Fig. 6

Effects of hsa_circ_0004781 on miR-9-5p and miR-338-3p target gene expression in PDAC cells. (A, B) Potential target mRNAs of (A) miR-9-5p and (B) miR-338-3p were predicted using mirDIP, miRTarBase, and TargetScan. Five upregulated targets were selected on the basis of the findings of GEPIA2 database analysis. (C) AsPC-1 and BxPC-3 cells were transfected with control siRNA (si-Ctr) or hsa_circ_0004781 siRNA (si-c4781) for 24 h. (D) AsPC-1 and BxPC-3 cells were transfected with a control vector (OE-Ctr) or pcDNA-ZKSCAN-c4781 plasmid (OE-c4781) for 24 h. (C, D) Total RNA was extracted, and the expression of miR-9-5p and miR-338-3p target mRNAs was determined through RT-qPCR. Values are presented as the mean ± SE from three independent experiments. *p < 0.05, **p < 0.01, ##p < 0.0001 vs. si-Ctr or OE-Ctr

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