Fig. 7

Effects of miR-9-5p and miR-338-3p on target gene expressions in PDAC cells. (A, B) AsPC-1 cells were cotransfected with the pcDNA-ZKSCAN-c4781 plasmid (OE-c4781) and either the control mimic (Ctr mimic), miR-9-5p mimic (miR9 mimic), or miR-338-3p mimic (miR338 mimic) for 24 h. Total RNA was extracted, and the expression of (A) miR-9-5p and (B) miR-338-3p target mRNAs was determined through RT-qPCR. Target gene expression levels were normalized to those of the control (no treatment). Values are presented as the mean ± SE from three independent experiments. *p < 0.05, **p < 0.01 vs. the Ctr mimic. (C) AsPC-1 cells were transfected with mimics or inhibitors of miR-9-5p and miR-338-3p for 24 h. Total cell protein lysates were collected, and the protein expression of KLF5 and ADAM17 was determined through Western blot analysis