Fig. 3
From: Doxorubicin loaded exosomes inhibit cancer-associated fibroblasts growth: in vitro and in vivo study
Dox loading confirmation. (A) Dox standard curve. Emission and excitation were determined using a fluorimeter at 595 and 480 nm, respectively. (B) Characterization of Exo-Dox using TEM images. (Bi) TEM image of Msc-derived exosomes. (Bii) The lipid membrane and integrity of exosomes after Dox loading are clearly visible, scale bar = 200 nm. (Biii) color change to dark gray could be observed for Exo-Dox, scale bar = 100 nm. (C) Fluorescent microscopy images of the drug-loaded Exo intracellular distribution. Blue fluorescence indicates the cell nucleus and red fluorescence indicates Exo-Dox scale bar = 50 μm (Ci) magnification, 20X. (Cii) magnification, 40X. The figures are based on three independent experiments