Fig. 10
From: Targeting the USP7-CDK1 axis suppresses estrogen receptor-positive breast cancer progression
Effect of inhibition of USP7 and CDK1 on ERPBC in ex vivo primary cell and in vivo xenograft models. (A) Eight-week-old female SCID mice were subcutaneously injected with MCF7 and T47D breast cancer cells. Tumors were excised (six mice per group) on day 50 after euthanizing mice. (B & C) Tumor sizes and body weights were measured twice per week and calculated using the formula V = (L × W2) / 2. Statistics are represented as mean volume ± SD (D) Primary ERPBC cells were cotreated with GNE-6776 and Ro-3306 for the MTT assay. (E) Apoptosis levels of primary ERPBC cells treated with GNE-6776 and Ro-3306 were analyzed via an Annexin V apoptosis assay. (F) Quantitative analysis of apoptosis induced by co-treatment of GNE-6776 and Ro-3306 in primary cells (G) A graphic model for the findings in this report