Fig. 4

Identification of CAF subtypes and evaluation of their marker gene expression. A, B The UMAP plot of the NCRT samples before/after batch effects corrected using the harmony. C The UMAP plot of six fibroblast clusters. D According to the marker gene expression, the distribution of CAF subtypes in samples were visualized by using the UMAP plot. E Heatmap of the top5 marker gene expression of each subtype. F The subtype distribution in the NCRT samples. G Bubble diagram of the top5 marker gene expression of each subtype. H, I Bubble diagram and distribution map of the 7 key marker gene expression in each subtype