Fig. 5

Loss of miR 2355-5p alters the ability of cells to stimulate angiogenesis. A-B Tube formation assays performed with CM from 786-0, 786-0 Cr.CTRL, 786-0 Cr.2355-2B, and 786-0 Cr.2355–2 C cells (N = 3). Image illustration (A) and quantification (B) of the number of tube-like structures (left) and total tube size (right) under each experimental condition. C-D Tube formation assays performed with CM from A498, A498 Cr.CTRL, A498 Cr.2355–2 A, and A498 Cr.2355-2B cells (N = 3). Image illustration (C) and quantification (D) under each experimental condition. Positive (10% FBS) and negative (0.5% FBS) controls were used. (Scale bars = 200µM) E-F Proteome Profiler Human Angiogenesis Array Kit used with CM from 786-0 Cr.CTRL and 786-0 Cr.2355–2 C cells. Image illustration from low-exposure (E) and high-exposure (F) and quantification of altered observable proteins. G-H Angiogenesis Array used with protein cell lysate from 786-0 Cr.CTRL and 786-0 Cr.2355–2 C cells. Image illustration from low-exposure (G) and high-exposure (H) and quantification of altered observable proteins. Each protein is represented by two dots and quantified as Mean. I-J RT-qPCR quantification of selected angiogenic factors in 786-0 Cr.2355 models (I) and A498 Cr.2355 models (J) compared to appropriate control cells (N = 3). Gene expression was normalized with RPLPO. The data are presented as the Mean ± SEM. Statistical analysis was performed using two-tailed unpaired Student’s t-tests (*P < 0.05, **P < 0.01)