Fig. 4

DUSP4 alleviates the phosphorylation of PGK1 at Ser203 and reduces the lactate production in OC cells. (A) Differential expression of PGK1 S203 in DUSP4-overexpression and vector-loaded OC cell lines detected by LC-MS/MS. (B-C) Expression levels of PGK1 and PGK1 S203 in DUSP4-overexpression and vector-loaded OC cell lines detected by WB. And the quantitative analysis using the relative gray values of protein bands. (D) Co-localization expression of PGK1 S203 and MitoTraker in DUSP4-overexpression and vector-loaded OC cells using immunofluorescence. The scale bar is 50 μm. (E) Intracellular lactic acid content in DUSP4-overexpression and vector-loaded OC cells. (F) Lactic acid content in cell supernatant of DUSP4-overexpression and vector-loaded OC cells. (G) Histogram analysis of intracellular ROS levels measured by flow cytometry. (H) Geometric mean fluorescence intensity (GeoMean) of ROS levels. GeoMean values represent the overall ROS levels across the cell population for each experimental group. (I) Peak fluorescence intensity (Mode) of ROS levels. Mode values represent the most frequent fluorescence intensity within the population for each experimental group. For experiments above, the significance of difference is determined by two-sided t test; *p indicates statistical difference; the data are presented as mean ± SD