Fig. 3

T cell maturation status in the blood and tumor tumor tissue were quantified. (A) Gating strategy using CD45RA and CD27 expression are shown to determine effector memory (EM) (CD45RA^-, CD27^-), central memory (CM) (CD45RA^-, CD27⁺), naïve (CD45RA⁺, CD27⁺) and terminal differentiated EM (TEMRA; CD45RA⁺, CD27^-) on CD4⁺ and CD8⁺ T cells. (B). Maturation of CD4 (second row) and CD8 (third row) T cells were analyzed by flow cytometry in the peripheral blood of healthy donors (HD) and blood and tumor of TNBC, HER2 and Luminal B patients. Data are given as mean ± SD and significances calculated using Tukey’s multiple comparisons test. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001