Fig. 4
From: The m6A reader IGF2BP2 promotes pancreatic cancer progression through the m6A-SLC1A5-mTORC1 axis
SLC1A5 promotes pancreatic cancer cell proliferation and glutamine uptake through activation of the mTORC1 pathway. A. mRNA levels of SLC1A5 in PANC-1 and PaTu 8988 cells determined by RT-PCR. B. Viability of PANC-1 cells and PaTu 8988 cells with or without SLC1A5 knockdown analyzed by the CCK8 assay. C. Representative images from the colony-forming assay and colony number analysis as indicated. D. Depletion of SLC1A5 inactivated mTORC1 signaling by western blot. E. Detection of intracellular glutamine levels through ELISA. **P < 0.01, ***P < 0.001, ****P < 0.0001