Fig. 5

Flow cytometry analysis of CRC stemness markers expression. The expression of CRC-CSC markers in HT-29 and Caco2 subpopulations exposed to 5-FU was analyzed using flow cytometry in comparison to their parental and spheroid cells. The changes in marker expression were confirmed by flow cytometry, specifically in CD44 and CD133 in Caco2 subpopulations exposed to 5-FU (A, C) and in CD44 in HT-29 subpopulations exposed to 5-FU (B, D). Data are presented as mean ± SD as *** = p-value < 0.001 and **** = p-value < 0.0001