Fig. 8

Wound scratch assay for migration assessment. Images of wound areas for Caco2 (A) and HT-29 (B) cells at 0 h and 24 h post-scratch are shown. The cell areas are highlighted in green, with the mean scratch length marked by a red line. The bar graphs on the right represent the percentage of the remaining scratch area at 0 and 24 h. For both Caco2 and HT-29 cells, 5-FU exposed CRC cells exhibited significant wound closure, indicating higher migratory capacity. However, migration capacity of parental and spheroid cells was insignificant. Data represent mean ± SD from two independent experiments. Statistical significance: *p < 0.05 and **p < 0.01