Table 1 Real-time PCR primer sequences
Genes groups | Gene name | Primer Sequence F: 5’ → 3’ R: 3’ → 5’ |
|---|---|---|
Housekeeping gene | GAPDH | F-CATGAGAAGTATGACAACAGCCT R- TGAACCAATGCAACCTTCTCGA |
Stemness genes | C-MYC | F-ACACATCAGCACAACTACG R- CCTCTTACGTTCACTCCG |
KLF4 | F-CCTCGCCTTACACATGAAGAG R- AAAGTGTGACAGAAAGGGCTAC | |
SOX2 | F-AATGGGAGGGGTGCAAAAGAGG R- GTGGTATTGGGATGGTGTGAGT | |
NANOG | F-AGCTACAAACAGGTGAAGAC R- GGAAATGAGAAGGGTGGTAG | |
OCT4-A | F-GTGGAGAGCAACTCCGATG R- GTCCTGATTTGCAGAGCTGT | |
EMT genes | Vimentin | F-TCTACGAGGAGGAGATGCGG R- CADAGAGCCGTCAAGTCGG |
SNAIL1 | F-CCAGAGTTTACCTTCCAGCA R- AGCAGGTTACGAGTAGAG | |
TWIST1 | F-TTCTCGGTCTGGAGGATGGA R- TAAGTTTCTTGCCGCCCAC | |
N-cadherin | F-GCCCAAGACAAAGAGACCC R- CAGTCAGTGAAGGAGTCAGC | |
E-cadherin | F-CAGGAGTCATCAGTGTGGT R- GACGTGGTTACGTTATTAGGAG | |
ZEB1 | F-CTTCTCACACTCTGGGTCTTATTC R- CATTCCTCTTCTCGCTCCAACG | |
ABC Transporter | ABCG2 | F-TTCCACGATATGGATTTACGG R- CCTGATGGCTTAAGTCCCTTG |
ABCB1 | F-GTTCAGGTGGCTCTGGATAAG R- CATTACGACTGACGATGCGA | |
ABCC1 | F-CGCCTTCGCTGAGTTCCT R- GTGTGGTGTGTCGCGT |