Fig. 6

PGAM1 was a Target of miR-135b-5p. A Putative binding sites between PGAM1 and miR-135b-5p. B, C Dual-luciferase reporter assay in miR-135b-5p-transfected or control OS cells. D, E RIP assays using antibodies against AGO2 or IgG were measured in cellular lysates from OS cells. F, G PGAM1 expression was detected in miR-135b-5p-transfected or control OS cells by western blotting and qRT-PCR. *p < 0.05; **p < 0.01; ***p < 0.001 (Student's t-test)