Fig. 2

Single-cell atlas of bone marrow cells across three groups (In vivo). (A) Flowchart illustrating the research process for single-cell RNA sequencing. (B) Thirty-four bone marrow cell clusters. UMAP representation of unbiased clustering of all bone marrow cells from the three groups (combined bone and bone marrow fractions, n = 5 mice). (C) UMAP representation of unbiased clustering and cell annotation for bone marrow cells from the three groups. A total of 12 cell types were identified. (D) Cluster signature genes are highlighted on the left. Average expression of the top differentially expressed genes (rows) across the cells (columns), with warmer colors indicating higher expression. (E) UMAP plot displaying the distribution of representative markers for each cell type. Color represents the relative expression level of each gene. (F) Proportions of each cell type