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Fig. 4 | Cancer Cell International

Fig. 4

From: Single-cell transcriptome sequencing reveals the mechanism of Realgar improvement on erythropoiesis in mice with myelodysplastic syndrome

Fig. 4

Arsenic disulfide promotes erythroid differentiation of mouse bone marrow cells in vitro. (A) Flowchart outlining the research process to investigate the effect of arsenic disulfide. (B) Mouse bone marrow cells were cultured for 48 h or 10 days (as indicated) with 625 nM As2S2. Expression of CD71 and Ter119 was assessed by flow cytometry. (C) Quantification of the percentages of CD71+Ter119+ populations shown in the left panel. Data are presented as mean ± SEM (n = 3). (D) K562 cells were cultured for 48 h (as indicated) with 30 μM hemin or 625 nM As2S2. Expression of CD71 and CD235a was analyzed by flow cytometry. (E) Quantification of the percentages of CD71+CD235a.+ populations shown in the left panel. Data are presented as mean ± SEM (n = 3)

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Cancer Cell International

ISSN: 1475-2867

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