Fig. 8

Preliminary validation of bioinformatics results. (A) Violin plot depicting the expression levels of S100a8 and S100a9 in erythroblasts. Gene expression in the WT group is shown in blue, in the NHD13 group in red, and in the Realgar group in green. Statistical significance is indicated above each gene. (B) Western blot analysis of S100a8 and S100a9 protein expression in Ter119⁺ bone marrow cells. (C) Quantification of the gray values of the protein bands in panel B. Data are expressed as mean ± SEM (n = 3). (D) Western blot analysis of ERK1/2 and pERK1/2 protein expression in mouse bone marrow cells after in vitro culture. (E) Quantification of the gray values of the protein bands in panel D. Data are expressed as mean ± SEM (n = 3). (F) Flow cytometric analysis of CD71 and CD235a expression in erythroblasts. (G) Quantification of the percentage of CD71⁺CD235a⁺ populations from the flow cytometry analysis in panel F. Data are expressed as mean ± SEM (n = 3)